Therefore, deciphering resistance genes and understanding their role in CCR resistance would help facilitate the development of resistant varieties.
The use of resistant varieties would be the most robust and economically viable way to control this disease. Both curative (pyraclostrobin, strobilurins, and azoxystrobin) and preventive (captan) fungicides are commonly used to control CCR, but chemical controls are not very effective once plants become infected ( MacKenzie et al., 2009 Rahman and Louws, 2017). Most commercial cultivars currently grown in the United States are susceptible to CCR and, thus, economic losses occur nearly every season from this disease. The necrotic infections in crown tissues cause necrosis, plant collapse, and death in hot and humid conditions ( MacKenzie et al., 2006 Rahman and Louws, 2017). Colletotrichum gloeosporioides is a necrotrophic fungi that causes Colletotrichum crown rot (CCR) of strawberry ( Fragaria × ananassa), which is an important disease in Florida and throughout the southeastern United States. These newly developed subgenome-specific functional markers for FaRCg1 can facilitate development of resistant varieties through marker-assisted selection.Ĭolletotrichum species are some of the most notorious and damaging diseases of the cultivated strawberry worldwide ( Münch et al., 2008). Significant and positive associations were found between the high-resolution melting (HRM) marker genotypes and CCR phenotypes. Subgenome-specific markers developed for the candidate genes were tested with a diverse panel of 219 accessions from University of Florida and North Carolina State University breeding programs. Three genes in the genomic region of FaRCg1 were highly upregulated: a von Willebrand Factor A domain-containing protein, a subtilisin-like protease, and a TIFY 11A-like protein. Gene ontology analysis showed that the most upregulated genes were functionally associated with signaling pathways of plant defense responses. In ‘Florida Elyana’, 6,099 genes were differentially expressed in response to C.
Here, we performed comparative transcriptome analyses of resistant ‘Florida Elyana’ and susceptible ‘Strawberry Festival’ after infection and identified candidate genes potentially involved in resistance. However, the genomic structure of FaRCg1 and potential candidate genes associated with the resistance remained unknown. Our previous study reported that a major locus, FaRCg1, increases resistance.
Saket Chandra 1 †, Youngjae Oh 1 †, Hyeondae Han 1, Natalia Salinas 1, Ashlee Anciro 1, Vance M.
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